A simplified genomic DNA extraction protocol for pre-germination genotyping in rice
نویسندگان
چکیده
منابع مشابه
A simplified genomic DNA extraction protocol for pre-germination genotyping in rice.
Genotyping is a critical step for molecular marker-assisted selection in rice. Rice genomic DNA samples for genotyping are typically isolated from living tissues such as seedlings. This requires the germination of all candidate seeds and extraction of DNA from the seedlings. Currently, an ideal individual is selected from a very large number of plants, which is time- and labor-consuming, requir...
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The presence of metabolites has been observed to create interference with DNA isolation procedures and downstream reactions, such as, DNA amplification, restriction and cloning. Moreover, the chemotypic heterogeneity among species may cause hindrance in optimal DNA yields with a single protocol. Thus, even closely related species may require different isolation protocols. A rapid and high yield...
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The presence of organic compounds and high amount of secondary metabolites (polysaccharides, phenolic component, etc.) in mosses cause difficulties in DNA extraction that are followed by problems in PCR reactions. In lower plants, various methods have been used for DNA extraction including silica gel and different commercial kits. These methods mostly use hazardous (like phenol or liquid nitrog...
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ژورنال
عنوان ژورنال: Genetics and Molecular Research
سال: 2015
ISSN: 1676-5680
DOI: 10.4238/2015.june.11.12